Thursday, 17 October 2019

Models questions for NET and other entrance exams


Model questions
1.      QTL stands for Quantitative trait loci
2.      Who coined the term quantitative trait loci – Gelderman (1975)
3.     
Used for the identification of fungal species
NTS stands for Non transcribed region               
4.      ITS stands for Internal transcribed spacer
5.      Expand ISEM- Immuno-sorbent electron microscopy (developed by Derrick)
6.      Sterilization temperature – 121.6o C for 15psi for 15-20min
7.      Mention any 2 surface sterilizing agents – Mercuric chloride (0.1%), Sodium hypochloride (1%)
8.      Expand ELISA – Enzyme linked immuno sorbent assay
9.      ELISA was developed by – Voller (1976), Clark and Adams (1977)
10.  Sensitivity of ELISA – 1-10 ng/ml
11.  Sensitivity of PCR – 1-10 pg/ml
12.  Substrate used in ELISA – Para Nitrophenol phosphate (PPP)
13.  Plates used in ELISA is made of Polystyrene plate
14.  Most sensitive technique used for the detection of Plant pathogens – ELISA
15.  No of wells present in ELISA plate - 96
16.  DNA barcoding was proposed by – Paul Hebert’s (2003)
17.  ISTA stands for – International seed testing association
18.  Blotter paper method was developed by – Louis Doyer (1938)
                                           Northern blotting (RNA)
                                                             Alwine, 1971

Western blotting
 (Protein)  Burnette, 1971
                                           Southern blotting (DNA)
                                                      E. M. Southern, 1973
·         Western blotting procedure was given by – Towbin, 1979
 


                                                               









19.  DIBA stands for – Dot immunobinding assay
20.  DIBA was developed by – Goodwin and Bantarri (1985)
21.  Substrate used in DIBA – BCIP -5 Bromo -4-chloro, 3-indolyl phosphate and
                                                          NBT – Nitroblue tetrazolium
22.  Plate used in DBIA – Nitrocellulose plate
23.  PCR was discovered by – Karry Mullis (1983)
24.  Substrate used in PCR – Taq polymerase (Thermus aquaticus)
25.  Standard extension temperature in PCR - 720C
26.  Expand RAPD – Random amplified polymorphic DNA (Williams)
27.  Expand RFLP – Restriction fragment length polymorphism (Bolstein)
28.  Expand PAGE – Polyacryl amide gel electrophoresis
29.  Expand LAMP – Loop mediated isothermal amplification (Notomi, 2000)
30.  PTGS (RNAi interference) was described by – Andrew Fire and Craig Mello (1998), got noble in 2006
31.  PTGS first described in the organism – Caenorhabditis elegans (nematode)
32.  DNA barcoding was proposed by – Paul Hebert (2003)
33.  Father of Indian mycology and plant Pathology – E. J. Butler
34.  1st imperial mycologist in India - E. J. Butler
35.  Founder of Indian Phytopathoogical society – B. B. Mundkur (1947)
36.  Fung and diseases in plants was written by - E. J. Butler,1918
37.  Fungi and Plant Diseases was written by - B. B. Mundkur, 1948
38.  Monograph on Indian Ustiaginales was written by - B. B. Mundkur
39.  Name the 1st pathogenic fungus completely genome sequenced – Magnoporthe grisea (Daen et al., 2005)
40.  Name the 1st pathogenic bacterium completely genome sequenced – Xylella fastidiosa (A. J. G. Simpson et al., 2000)
41.  Name the 1st pathogenic virus completely genome sequenced – Cauliflower mosaic virus (Frank et al., 1980)
42.  Name the 1st pathogenic RNA virus completely genome sequenced – TMV (Goelet et al., 1982)
43.  Who coined gene for gene hypothesis – H. H. Flor
44.  Protein for protein hypothesis – J. E. Vanderplank
45.  Father of  epidemiology - J. E. Vanderplank
46.  What is the 1st phytoalexin – Pisatin
47.  Who coined the term phytoalexin – Muller and Borger (1940), worked on potato and Phytophthora infestans
48.  Phytoalexin is a greek word which means plant protecting substance
49.  Father of seed pathology – Paul Neergaaurd
50.  Who coined the term Seed Pathology - Paul Neergaard and Mary noble
51.  Internally seed borne fungi Wheat loose smut (Ustilgonuda tritici),
                                                     Loose smut of barley (Ustilagonuda hordei)
52.  Externally seed borne fungi – Bunt of rice (Neovassia horrida),
                                                     Covered smut of jowar (Sphacelotheca sorghi) and
                                                     Covered smut of barley (Ustilago hordei)
53.  What is the resistant race of Puccinia graminis f.sp. tritici (UG99, present resistant Sr-31)
54.  Who coined the term appressorium – A. B. Frank
55.  Foolish seedling diseases is otherwise called as – Bakane disease
56.  Killer disease of rice – Tungro disease
57.  Fusarium producing mycotoxins – Deoxynivelonol, Fumonisins and Trichothecins
58.  Phenolic theory was given by – J. C. Walker
59.  Father of molecular plant pathology – Noel Kein
60.  Who coined the term elicitor – Noel Kein (1975)
61.  Irish famine – Phytophthora infestans
62.  Bengal famine – Bipolaris oryzae
63.  Molecular koch pastulates was given by – Stanley Falkow
64.  Who coined the term Vivotoxin – Dimond and Waggoner (1953)
65.  Phytoalexins
Bean – Phaseollin
Pea – Pistin
Potato - Rishitin
Sweet potato – Ipomeamarone
           
                                          Fungi
66.  Father of mycology – P. A. Michelli (book – Nova Plantarum Genera)
67.  Linnaeus of mycology – E. M. Fries
68.  Reconstructor of mycology – L. R. Tulsane
69.  Father of forest pathology – Robert Hartig
70.  Father of tropical plant pathology – H. M. Ward
71.  Father of botany – Theophrastus
72.  Great grandfather of Phytopathogy – M. Tillet
73.  1st book completely devoted to fungi – Theatrum fungorum (Van sterbeeck, 1675)
74.  1st text book in plant pathology –The Diseases of cultivated crops and Their causes and Their control (J. G. Khun, 1858)
75.  J. G. Kuhn also considered as microscopic manager
76.  Sexual stage of rust fungi (Pycnia) was discovered by – Craigie, 1927
77.  Heterothallism was discovered by – A. F. Blackslee, 1904 (Rhizopus stolonifer)
78.  Temperature requirement of Yellow rust 10 - 15 o C
                                                      Leaf rust 15 – 20 o C
Stem rust 20 – 30 o C
·         Yellow rust/ stripe rust – Puccinia striiformis
·         Leaf rust/ brown ruts – Puccinia recondita/ P. triticina
·         Stem rust/ black rust – Puccinia graminis f. sp. tritici
79.  Origin of physiological races through heterokaryosis was reported by – Hansen and Smith (1932)
80.  Heterokaryosis was reported in which organism – Botrytis cinerea
81.  Heterokaryosis – fusion of 2 different kinds of nuclei
82.  Parasexuality was discovered by – Pontecarvo and Roper (1952)
83.  Parasexuality was observed in – Aspergillus nidulans
84.  Hartig nets re found in – Ectomycorrhizae
85.  Muriform conidia found in – Alternria (muriform – having both horizontal and vertical septations)
86.  Pyriform conidia found in – Pyricularia (Pyriform - pear-shaped, number of cells swollen at one end resembling a tennis racquet)
87.  Flame shaped conidia is produced by – Venturia (browm, 2 celled)
88.  Sickle shaped conidia – Colletotrichum
89.  Filiform shaped ascospore – Claviceps (Scolecospore)
90.  Filiform conidia is produced - Mycosphaerilla
91.  1st Indian studied and started to collect fungi – K. R. Kritikar


Asexual spores
Sexual spores
Mastigomycotina
Zoospore
Oospore
Zygomycotina
Sporangiospore, Chlamydospore , Oidia
Zygospore
Ascomycotina
Conidia
Ascospore
Basidiomycotina
Conidia
Basidiospore

92.  Robigola festival is celebrated on – April 25

93.  Alternate names

Zygomycota
Yolk fungi
Ascomycota
Sac fungi
Basidiomycota
Club fungi
Deuteromycota
Dustbin fungi/ fungi imperfecti

94.  Soft rot of sweet potato – Rhizopus stolonifer
95.  Soft rot of squash – Choanephora cucurbitarum
96.  Bread mould – Rhizopus niger
97.  Pin/ red bread mould – Neurospora crassa
98.  Peach leaf curl – Taphrina deformans
99.  Withes broom of cherries – Taphrina ephiphyla
100.                      Citrus scab is caused by – Elsinoe fawcetti
·         Grapes anthracnose is caused by – Elsinoe ampelina
·         Raspberry anthracnose is caused by – Elsinoe veneta
·         Rice sheath (cobra diseases of rice) blight is caused by – Rhizoctonia sloani
·         Spindle shaped ascospore is produced by – Spermophthora gossypii
·         Hat shaped ascospore is produced by – Ascoidea rubescens  
·         Leaf rust of rice – Entyloma oryzae
·         Crozier formation can be seen in – Ascomycota (restricted dikarophage)
·         Clamp connection can be seen in – Basidiomcota (extended diaryophage)
·         Green muscardian fungi – Metarhizium anisipliae
·         White muscardian fungi - Beauveria bassiana
·         Budding yeast - Sacchromyces cerevisiae  
·         Fission yeast – Schizosacchromyces
·         Weed of the fungal world – Aspergillus niger
·         Aflotoxin is produced by – Aspergillus flavus/ Aspergillus parasiticus/ Aspergillus nominus
·         Colony colour

Aspergillus niger
Black
Aspergillus flavus
Green
Aspergillus tamari
Brown
Aspergillus ochrceus
Yellow 
Aspergillus candidus
White

·         Candle snuff fungus – Xylaria hypoxylon
·         Dead man’s finger – Xylaria polymorpha
·         False morels – Helvella 
·         True morels -  Morchella
·         Podosphaera leucotricha produces -  1 ascs
·         Hypocrea/ Rufa produces – 16 ascospores (Hypocrea is otherwise called as Rufa)
·         Podospora produces 512 ascospores
·         Ballistospores – actively discharged spores (forcily discharged)
·         Strastismospores – Passively discharged spores
·         White pine blister blight is caused by – Cronartium rubigola
·         Repeating spores – Uredospores
·         Resting spores – Teliospores
·         Alternate host for Black stem rust – Barberry and Mahonia
        Brown rust – Thalictrum and Isopyron
        Bajra stem rust - Brinjal
        Jowar/ Sorghum rust – Oxalis corniculata (sleeping beauty)
·         Demicyclic rust means – Lack of uredial stage
(eg – cedar apple rust – Gymnosporangium juniper-viriginianae)
·         Appresorium – attachment organ
·         Haustoria – nutrients absorbing organ
·         Who 1st successfully reported axenic culture of cedar apple rust – Cutter (1951)
·         Who 1st successfully culture the axenic culture of Puccinia graminis triticiWilliams (1966)
·         Single celled teliospore – Uromyces
·         Double celled teliospore - Puccinia
·         Multicelled teliopsores – Phragmidium
·         Kidneyshaped uredospore/ Durnip  shaped teliospore – Hemileia (humped beacked/ renifrom shape)
·         Crust like teliospore – Melampsora
·         House fungus – Serpula lachrymans
·         Coral fungus – Claviceps sp
·         Fairy rings fruiting bodies are formed by -  Agaricus
·         Fly fungus – Entomophthora  and Amanita muscaria
·         Earth ball fungi – Scleroderma
·         Earth star fungi – Geastrum
·         Give an example of phototrophic fungus – Pilobolus, Sardaria, Peziza
·         Ulcer like lesions is produced by – Anthracnose (Diplocarpon, Elsinoe & Glomerella)
·         Diamond shaped symptoms produced by – Pyricularia oryzae
·         Target board symptoms produced by– Alternaria
·         Spore ball farming smut genera  - Urocystis, Sporisorium and Tolyposporium
·         Geniculate conidia – Cercospora sp
·         Jet black conidia -  Nigrospora oryzae
·         Fusarium producing toxins – DON (Deoxynivelanol) Fumonisins, Trichothecene, Zearalenone, Fusaic acid









Asexual stage/ Anemorph 
Sexual stage/ teleomorphic stage
Aspergillus
Eurotium
Penicillium
Talaromyces
Colletotrichum
Glomerella
Fusarium
Gibberella
Trichoderma
Hypocrea
Verticiliium
Hypocrea
Bipolaris
Cochiliobolus
Alternaria
Lewia
Drechslera
Pyrenophora
Botrytis
Botrytinia
Monilia
Monilinia
Rhizoctonia
Thanatephorous
Sclerotium
Aethalium

·         Mushrooms

White button mushroom
Agaricus bisporus
Oyster mushroom
Plerotus sojar-caju
Paddy straw mushroom
Volvariella volvaceae
Shiitake mushroom
Lentinus edodes
Milky mushroom
Calocybe indica
·         Death cap – Amanita muscaria (world’s deadliest mushroom, lethal dosage 0.1mg/kg of body weight)
·         Destroying angelAmanita verna/ Amanita virosa
·         Example of holocarpic thallus - Olpidium and Synchytrium
·         Example of eucarpic thallus – Pythium and Phytophthora
·         Fungi cell wall is made up of – Chitin
·         Oomycota cell wall is made up of – Cellulose + hydroxyproline
·         Zygomycetes cell wall is made up of  - Chitosan chitin
·         Yeast cell wall is made up of – Mannin β glucan
·         Mucor is otherwise called as – Pin mould (stolons and rhizoids present)
·         Rhizopus otherwise called as – Bread mould (stolons and rhizoids absent)
·         Fungi used for genetic studies – Neurospora crassa, aspergillus
·         Bacteria used for genetic studies – Agrobacterium
·         Virus used for genetic studies – CaMV, CMV, TMV
·         Inverted T shaped foot cells are present in – Aspergillus (absent in penicilium)

Disease
Pathogen
Nature
Spore viability
Grain smut
Sphacelotheca soghi
Externally seed borne
>10yrs
Head smut
Sphacelotheca reliana
Seed and soil borne
2 yrs
Loose smut
Sphacelotheca cruenta
Externally seed borne
4 yrs
Long smut
Tolyposporium ehrenbergi
Air borne
2 yrs


Bacteria
1.      Who coined the term bacteria – Ehrenberg
2.      Who 1st discovered bacteria – Antonie van Leeuwenhoek
3.      Father of microbiology - Antonie van Leeuwenhoek
4.      Founder of modern bacteriology – Louis Pasteur
5.      Father of bacteriological techniques – Robert Koch
6.      Father of plant bacteriology – E. F. Smith
7.      Father of Indian bacteriology – M. K. Patel
8.      Who reported the 1st bacterial disease – T. J. Burill (1878)
9.      What is the 1st bacterial disease to be reported – Fire blight of apple (Erwinia amylovora)
10.  Who coined the term chemotherapy – Paul Ehrlich
11.  Who coined the term genophore – Ris (1961)
12.  Who started the school of agriculture in pune – M. K. Patel
13.  M. B. Waite (1891) – proved that insect helps (bees and wasps)
14.  What is the first pant bacterial disease reported in India – Bangle blight of potato/ brown rot of potato (Ralstonia solanacearum)
15.  Length of bacteria – 0.5 to 1.0 µm - 2.0 to 5.0 µm
16.  Conformation test for gram staining was given by – Suslow (1952), 3 % KOH
17.  Bacterial avirulent gene was 1st demonstrated by – Staskawics (1984)
18.  Fungal avirulent gene was 1st demonstrated by – Dewitt
19.  Bacterial avirulent gene – Pseudomonas syringae pv. glycenia (avrD, Avr 6)
20.  Fungal avirulent gene – Cladosporium fulvum (CF 9)
21.  1st avirulent gene to be discovered – Bacterial avirulent gene (soybean), then after fungal avirulent gene
22.  Who introduced the pathovar system – Dye et al (1980)
23.  Concept of bacterial hypersensitivity – Klement (1964)
24.  Concept of fungal hypersensitivity – Stackmann (1915)
25.  Concept of viral hypersensitivity – Holmes (1929)
26.  Gram staining
·         Primary stain – crystal violet
·         Mordant – iodine solution
·         Decolorizing agent – 95% alcohol
27.  fluctuation test in bacteria – Luria and Delbruck (1940)
28.  fluctuation test is used for the identification of – Bacterial mutation
29.  Penicillin was discovered by – Alexander flemming (1928)
30.  Streptomycin was discovered by – Waksmann
31.  Bacterial cell wall is made up of – Mucopeptides/ peptidoglycon
32.  Who proved that presence of murein as bacterial cell wall – Weidal (1960)
33.  L form bacteria – cell wall lacking bacteria (Agrobcterium and Erwinia)
34.  V shaped bacteria – Corynebacterium
35.  Endospore staining agent – Malachite green
36.  Capsule staining agent – India ink
37.  Murein A - Consists of β 3, 4 linkage
38.  Murein B - Consists of β 2, 4 linkage
39.  How many rings in gram positive – 2 rings (M, S)
40.  How many rings in gram negative – 4 rings (L, P, M, S)
41.  B’ dellovibrio – bacteria that affect another gram negative bacteria (Stlop, 1962)
42.  Conjugation – E. coli (Lederberg and Tatum, 1946)
43.  Transformation – Pneumonia (Griffith, 1928)
44.  Transduction – Solmonella (Lederberg and Zinderberg, 1952)
45.  Five kingdom classification – Whittaker (1969)
46.  Six kingdom classification – Carl Woose (1977)
47.  Xylem inhabiting bacteria – Xylella fastidiosa and Clavibacter
48.  Phloem inhabiting bacteria – Serratia marcescens and Candidatus liberibacter
49.  Endospore farming gram positive bacteria – Bacillus and  Clostridum
50.  Size of Ti-plasmid – 200 kb
51.  Size of T- DNA – 15-24kb
52.  Endospore forming bacterial genus – Bacillus, Clostridium etc
53. 
Leafy gall disease – Rhodococcus fascians                                          causes out growth Olive knot disease – Psuedomonas savastanoi pv. savastanoi       in plants
54.  Vector for bacterial wilt of corn – Corn flea beetle (Chaetonema pulicaria & C. denticulata)
55.  Vector for vascular wilt of cucurbits – Striped cucumber beetle (Diabrotia vittata)
                                                                Spotted cucumber beetle (D. duodecimpunctata)
56.  Most of the plant pathogenic bacteria are – Rod shaped (g-ve)
57.  The term plasmid was coined by – Lederberg (1952)
58.  Hydathodes – Black rot of cabbage
                           Bacterial leaf blight of rice 
59.  Stomata – Bacterial leaf streak
60.  Trichomes - Bacterial canker of tomato
61.  Lenticels – Potato scab
62.  Atrichous – Xylella
63.  Monotrochous – Xanthomonas
64.  Amphitrichous – Pseudomonas spp
65.  Peritrichous – Erwinia
66.  Cephalotrichous – Pseudomonas fluorescens
67.  Bergey’s manual of determinative bacteriology was published in – 1923
68.  Bergey’s manual of systemic bacteriology was published in – 1984
69.  2nd edition of Bergey’s manual of systemic bacteriology was published in – 2004
70.  Elimination of plasmid is known as – Curing
71.  Agents used for the elimination of plasmid – Acridine dye, heat and Uv light 
72.  AgrobacteriumConn (1942)
73.  ClavibacterDavis (1984)
74.  ErwiniaWinslow (1920)
75.  PseudomonasMigula (1894)
76.  XanthomonasDowson (1939)
77.  Bacterium mostly used for horizontal gene transfer – Agrobacterium
78.  Average speed of bacteria – 30 – 50 µm/ sec
79.  Mode of action of phseolotoxin (Pseudomonas syringae pv. phaseolicola) – inactivates the enzyme carbamoyltransferase
80.  Mode of action of Tabtoxin (P. syringae pv. tabaci) – Inhibits glutamine synthatase
81.  Protoplast – totally damaged cell wall (g+ve)
82.  Spheroplast – partially damaged cell wall (g-ve)
83.  PPLO – no cell wall
84.  ConjugationE. coli - Lederberg and tatum - 1946
85.  TransformationPneumonia – Griffith - 1928
86.  TransductionSolmonella – Lederberg and zinderberg - 1952
87.  Test used to identify bacterial diseases – Ooze out test
88.   Gram stating was developed by – Christian gram (1884)
89.  L form of bacteria means – lack of cell wall
90.  L form of bacteria was isolated by – Klieneberger nobel (1935)
91.  Exotoxins are found in –  both g-ve and g+ve
92.  Endotoxins are found in – g-ve
93.  Bacteriophage was discovered by – Twort and Herelle (1915, 1917)
94.  Ralstonia solanacearum races
                                           Race 1 – Solanaceae
                                           Race 2 – Musaceae
                                           Race 3 – Potato
   Race 4 – Ginger
   Race 5 - Mulberry 
95.  Bacteria blight of pomegranate – Xanthomonas axinopodis pv. punicae
96.  Sugarcane ratoon stunting is caused by – Leifsonia xyli subsp. Xyli (transmitted by setts)


Plant virology
1.      Father of virology – Beijernick
2.      Who developed local lesion assay – Holmes
3.      TMV was 1st purified by – Stanley (1935) by using ammonium sulphate
4.      Viroid was discovered by – Diener and Raymer (1971)
5.      1st viroid diseases to be reported – potato spindle tuber viroid
6.      1st viroid disease in India – Tomato bunchy top viroid
7.      Cross protection technique was developed by – Mckinney (1929)
8.      Hybridoma technology for monoclonal antibody production was developed by – Kholer and Milstein (1975)
9.      ELISA was developed by – Voller (1976), Clark and Adams (1977)
10.  Phytoplasma – Doi (1967), Mulberry dwarf
11.  Spiroplasma – Davis (1972), Corn stunt diseases
12.  Satellite virus was discovered by – Kassanis (1962)
13.  Bimodal transmission was 1st reported by – Chalfant and Chapman (1962) on CaMV
14.  1st DNA virus to be completely genome sequenced – CaMV (Frank et al, 1980)
15.  1st RNA virus to be completely genome sequenced – TMV (Goelet et al, 1982)

Content
NA (Nucleic acid ) %
Protein %
All viruses
5 – 40
60 - 95
TMV
5
95
Turnip yellow mosaic virus
34
66

16.  Fovea virusApple stem pitting virus
17.  TrichovirusApple chlorotic leaf spot virus
18.  Largest plant virus – Rhabdovirus
19.  Largest plant virus family – Potyviridae
20.  Longest plant virus – CTV
21.  Smallest viroid – Coconut cudang cudang viroid (246)
22.  largest viroid – Chrysanthemum chlorotic mottle viroid (401)
23.  TMV
·         158 – amino acids
·         2130 – capsomere sub-units
·         6400 – Nucleotides
·         Measurement length – 300 × 18 nm
·         TIP (thermal inactivation point) – 93 o C
24.  Stain used for the detection of Phytoplasma – DAPI and Diene’s stain
25.  Genome of geminiviridae – ss DNA
26.  Geminiviridae divided into 4 genera

Genera
Type species
Transmission
Mastrevirus
Maize streak virus
Leaf hoppers
Curtovirus
Beet curly top virus
Leaf hoppers
Begmovirus
Bean golden mosaic virus
White flies
Topocuvirus
Tomato pseudo curly top virus
Tree hopper

27.  Cell to cell movement of virus is facilitated by – plasmodesmata
Genome
Virus group
Example
ss RNA
Most of plant viruses
TMV
ds RNA
Reovirus
Wound tumour virus (70nm)

Mycovirus

ss DNA
Geminivirus
Bean golden mosaic virus
ds DNA
Caulimovirus 
Cauliflower mosaic virus



                         Epidemiology
1.      Father of epidemiology – Vanderplank
2.      Book written by vanderplank – Plant diseases epidemics and control (1963)
3.      Principles of plant infection was written by – Gaumann (1946)
4.      Indian stem rust rules – Nagarajan and Singh (1975)
5.      Puccinia pathway – Nagarajan and Joshi (1978)
6.      Active spore dispersal –Pilobolus and Entomophthora muscae
7.      Passive spore dispersal – Botrytis cinerea and Heminthosporium sativum
8.      Gene for gene hypothesis – H. H. Flor (1946)
9.      Protein for protein hypothesis – Vanderplank (1982)
10.  AUDPC – Area under disease progress curve (vanderplank, 1963)
11.  Expert system
·         TOM –Tomato
·         CONSELLOR – wheat
·         CALEX – peach and nectarine
12.  Simple interest diseases/ monocyclic – Saturation curve (0.02units/ day)
13.  Compound interest diseases/ polycyclic – Sigmoid curve/ S shape (0.1 – 0.5 units/ day)
14.  Polyetic diseases – Bimodal curve (0.02 – 2.3 units/ yr)
15.  1st computer stimulation model – EPIDEM (early blight of tomato and potato), Waggoner and Horsfall, 1969
16.  Stem rust resistant genes – Sr2 & Sr36
17.  leaf rust resistant genes – Lr34 & Lr37
18.  Van Everdingen rules (1926)/ Dutch rules applicable for – Phytophthora infestans
19.  Mills rules are applicable for – apple scab
20.  CERCOS – Cercospora blight of celery
MYCOS – Mycosphaerella blight of chrysanthemum
EPIMAY – Southern corn leaf blight
EPIVEN – apple scab
EPIDEMIC – wheat stripe rust
BARSIM-I – barley leaf rust
EPIGRAM – barley powdery mildew
SIMPHYT – potato late blight
BLIGHTCAST – late blight of potato
FAST – Forcasting Alternaria solani of tomato
21.  Boom and bust cycle was given by – Priestley (1970)
22.  Stewart wilt of corn – Pantoea stewartii
23.  Pea root rot – Aphanomyces euteiches
        
  Phanerogamic parasites and Non parasitic diseases
Ø  Complete stem parasite – Cuscuta
Ø  partial stem parasite – Loranthus
Ø  Complete root parasite – Orabanche
Ø  Partial root parasite – Striga
Ø  Aeginetia pedunculataobligate root parasite (Holoparasite) of sugarcane
Ø  Aeginetia pedunculata- 1st reported in India by – Ray and Dasgupta (2004)
Ø  Witches broom of cherries is caused by - Taphrina epiphylla/ Taphrina wiesneri
Ø  Which Phanerogamic parasites having widest host range – Cuscuta
Ø  Widely distributed parasitic species in the word – Cuscuta campestris
Ø  Trap crop for Orobanche (Broom rape) – Linseed/ flax seed 
Ø  Dwarf mistletoe – Arceuthobium
Ø  True mistletoe – Phoradendron viscus
Ø  Silver leaf of tomato is caused by – PAN (Peroxyacyl nitrates)
Ø  Most destructive air pollutant to plants that is – Oozone (0.1 – 0.3 ppm)
    Nutritional deficiency

Sl.no
Disease/ disorder
Nutritional deficiency
1
Boron
Halo stem of cauliflower
Top sickness of tobacco
Fruit cracking of apple
Hard rot of citrus
Hen and chicken disorder of grapes
Heart rot of sugar beet
2
Calcium
Blossom end rot of tomato
Blossom end root of melon
Bitter pit of apple
3
Copper
Die back of citrus
4
 Iron
Green netting of citrus
Chlorosis in sugarcane
6
Manganese
Pahala blight of sugarcane
Marsh spot of pea
Grey speck of oats
Crinkle leaf disorder of citrus
7
Molybdenum
Whip tail of cauliflower
8
Nitrogen
Buttoning of cauliflower
Red leaf of cotton
9
Potassium
Cotton rust
Marginal necrosis of corn
11
Sulphur
Sulphur diseases of mango
Punsukh disease of rice
Yellowing of tea leaves
12
Zinc
Little leaf of citrus
Little leaf of mango
Rice khaira diseases
White tip of maize
Plant quarantine
Ø  EPPO- European and Mediterranean Plant Protection Organization
Ø  NAPPO- North American Plant Protection Organization
Ø  IPPC- International Plant Protection Convention
Ø  1st International Plant Protection Convention held in - London
Ø  Which country having toughest quarantine standards in the world – Australia
Ø  DIP (Destructive insects and pests) act – 1914
Ø  First quarantine law was promulgated in – France (1660)
Ø  2003 – Plant quarantine order
Ø  2011 – Online plant quarantine services
Ø  NBPGR -  National bureau of plant genetic resources
Ø  FRI – Forest research institute
Ø  BSI – Botanical survey of India
Ø  Phytosanitary certificate is otherwise known as – Phytos/ Rome certificate
Pathogens are introduced to India
Sl.no
Disease
Pathogen
Year
Country
1
Coffee Leaf rust
Hemileia vastatrix
1879,
Sri Lanka
2
Late blight of potato
Phytophthora infestans
1883
Europe
3
Downy mildew of grapes
Plasmopara viticola
1910
Europe (France)
4
Banana bunchy top
Banana bunchy top virus
1940
Sri Lanka
5
Potato wart
Synchitrium endobioticum
1953
Netherlands
6
Onion smut
Urocystic cepulae
1958
Europe
7
Downy mildew of onion
Peronospora destructor
1977
Europe
8
Fire blight Pear and Apple
Erwinia amylovora
1940
England

Ø  Plant pathogens that has not been reported in India
Fungi
Downy mildew of soybean
Peronospora manshurica
Ergot of wheat
Claviceps  purpurea
Blue mould of tobacco
Peronospora tabacina
Dwarf bunt o wheat
Tilletia controversa
Bacteria
Fire blight of apple
Erwinia amylovora
Stewart wilt of maize
Pantoea stewartii
Sugarcane gummosis
Xanthomonas compestris pv.vasculorum
Virus
Plum, peach
Plum pox virus
Sugarcane
Fiji virus
Cocoa
Cocoa swollen shoot virus
Ø  Stem eel warm – Ditylenchus dipsaci
Ø  DPPQS Directorate of Plant Protection, Quarantine and storage
Ø  AOA – Agreement on agriculture
Ø  TRIPs - Agreement on Trade related intellectual property rights
Ø  TBT – Technical barrier to trade
Ø  PFA Pest free area
List of pest covered under domestic quarantine regulation
Sl.no
Pest/ Disease
Host plant
Restricted from states
1
Banana bunchy top virus
Banana planting material
Assam, Kerala, Odisha, Tamil Nadu, and West bengal
2
Banana mosaic virus
Banana plants and  material
Maharashtra and Gujarat
3
Apple scab (Venturia inaequalis)
Apple planting material
Jammu and Kashmir
4
Potato wart (Synchitrium endobioticum)
Potato
West Bengal
5
Potato cyst nematode (Globodera rostochiensis)
Potato
Tamil Nadu
6
Coffee berry borer (Hypothenamus hampi)
Coffee 
T.N, Karnataka, Kerala
7
Flute scale (Icerya purchasi)
Many host
Karnataka, Chennai, Kerala
8
San jose scale (Aspidiotus perniciosus)
Many host
Punjab, UP, T.N, WB, Assam, Odisha, HP, Jammu and Kashmir
9
Codling moth (Carpocapsa pomenella)
Apple and Walnut plants including fruits
Ladakh district


Chemical nature and classification of fungicides
Ø  Bordeaux mixture was developed by – Millardet (1882)
Ø  Burgundy mixture was developed by – Masson (1887)
Ø  Chestnut compound was developed by – Bewley (1921)
Ø  Who proposed the zone of inhibition – Burlingham and Reddish
Ø  Who introduced first systemic fungicide – Van Schmeling & Marshal Kulka (1966), Carboxin
Ø  Mode of action of Carboxin – Succinate dehydrogenase inhibitor (respiration inhibitor)
Ø  Book written by Horsfall – Fungicides and their actions (1945)
Ø  Mode of actions
Chemical
Mode of action
Carboxin & Oxycarboxin
Succinate dehydrogenase inhibitor
Benzimidazoles
Inhibits beta tubulin bio synthesis
Metalaxyl
Disturbs the fungal nucleic acid synthesis
Cycloheximide
Inhibits protein synthesis
Chlorani & Dichloran
Disturbs the electron transport system

Ø  Sticker – substances which improves the retention of parties on plant surfaces
Ø  FRAC  - Fungicide resistance action committee (1981)
Ø  Fungicides acts as plant defence activator – Forsetyl Al, Metalaxyl and Probenazole
Ø  Sulphur sensitive crops – Cucurbits, Apple, Melon and Grapes
Ø  Protectant fungicides – Bordeaux mixture, Chorothalanil and Mancozeb
Ø  Fungicides with symplastic movement – Forsetyl Al, Metalaxyl
Ø  Most commonly used antibiotics – Oxytetracycline and streptomycin (50 – 300 ppm)
Ø  1st antibiotic used for plant diseases control - streptomycin (fire bight of pear)
Ø  Who coined the term antibiotic – Waksman and Henrici,  1943
Ø  1st antibiotic crystalized – Gliotoxin
Ø  1st commercial organic product – Uspulum
Ø  Who 1st used systemic fungicide in India – Charanth (1969) against wheat loose smut
Ø  Who 1st used copper fungicide for the control of sorghum rust – Ozanne (1885)
Ø  Who 1st used Bordeaux mixture in India – Lawrence (1904)
Ø  Who wrote silent spring book – Rachel carson (1962)
Ø  Captan – Kittleson Killer – 1952
Ø  Rust – Oxycarboxin – Plantvax
Ø  Smut – Carboxin – Vitavax
Ø  Book written by Y. L. Nene – Fungicides in plant disease control (1971)
Ø  Book written by S. C. Vyas – Systemic fungicides (1984)
Ø  Seed protectant-  Captan, Thiram, carbendazim and carboxin
Ø  Fruit protectant – Captan, maneb, carbendazim and mancozeb
Ø  Flower/ blossom protectant – Captan, ferbam, zineb, mancozeb and chlorothalanil
Ø  Soil protectant -  Bordeaux mixture, COC, captan and thiram

Biological control
Ø  Who reported the parasitic nature of Trichoderma lingnorumonWeindling (1932)
Ø  Father of biological control – S. D. Garret
Ø  Who coined the biological control – Von Tabuef (1914)
Ø  Father of soil microbiology – S. A. Waksman
Ø  Who introduced the concept of mycoherbicide – Daniel (1973)
Ø  1st registered mycoherbicide – DeVine (Phytophthora palmivora)
Ø  1st mycoherbicide – Collego (Colletotrichum gloeosporioides)
Ø  SAR (systemic acquired resistance) coined by – Ross (1960)- Salicylic acid
Ø  Induced systemic resistance (ISR) – Van loon/ Van peer (1991), Jasmonic acid, Ethylene 
Ø  Induced systemic resistance (ISR) was 1st describe in - Arabidopsis
Ø  Bacteriocin was discovered by – Andrew Gratia (1925)
Ø  Bacteriocin produced by E. coli -  Colicins
Ø  Mycoparasite – fungi living in association with another fungi
Ø  Bio-control agent for wheat leaf rust – Darluca filum
Ø  Bio-control agent for cucurbits powdery mildew, grapes powdery mildew – Ampelomyces quisqualis 10
Ø  Commercially available bio-control agents

Name
Source
Galltrol
Agrobacerium radiobacter K 84
Nogall
Agrobacterium radiobacter K 1026
Kodiak
Bacillus subtilis GB 03
Dagger G
Pseudomonas  fluorescens
AQ 10
Ampelomyces quisqualis 10
F- stop
Trichoderma harzianum
SoilGard
Gliocladium virens GL -21

Ø  Mycoherbicides

Trade name
Mycoherbicide
Weed controlled
Weed scientific name
Collego
Colletotrichum gloeosporioides
North jointvetch
Aeschynomene virginica
Biomal
Colletotrichum gloeosporioides
Round-leaf mallow
Malva pusilla
Devine
Phytophthora palmivora
Milk weed wine weed
Morrenia adorata
Casst 
Alternaria cassiae
Sickle pod
Senna obtusifolia
LUBOA 2
Colletotrichum goeosporiodes f.sp. cuscutae
Dodder
Cucuta sp
ABG 5003
Cercospora rodmnii
Water hycanith
Eichhorina crassipes

Ø  Who 1st coined the term fungistasis – Dobbs and Hinson (1972)
Ø  1st commercially available to control butt rot of pines – Peniphora gigantean/  Phlebiopsis gigantean
Ø  Who 1st developed the commercial bio-control agent for crown gall (Agrobacterium radiobacter), K84  - New and Kerr (1972)
Ø  What are the different antibiotics produced by TrichodermaGliotoxin, Trichodermin and viridin
Ø  Book written by Baker and Cook (1974) -  Biologic control of plant pathogens
Ø  1st plant defence activator compound – CGA 245704 (Actigard), 1996



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                                                           Model NET/ ASRB question papers  N.H. SHANKAR REDDY (Ph.D., Plant Pathology), (Yo...